Biphasic role of nuclear factor-kappa B on cell survival and COX-2 expression in SOD1 Tg astrocytes after oxygen glucose deprivation
- Authors
- Lee, YS (Lee, Yong-Sun); Song, YS (Song, Yun Seon); Giffard, RG (Giffard, Rona G.); Chan, PH (Chan, Pak H.)
- Issue Date
- Aug-2006
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, v.26, no.8, pp 1076 - 1088
- Pages
- 13
- Journal Title
- JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
- Volume
- 26
- Number
- 8
- Start Page
- 1076
- End Page
- 1088
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/148563
- DOI
- 10.1038/sj.jcbfm.9600261
- ISSN
- 0271-678X
1559-7016
- Abstract
- In cytoplasm, nuclear factor-kappa B (NF-kappa B) is associated with the inhibitory protein, I kappa B alpha. On activation by H2O2, I kappa B alpha is phosphorylated and degraded, exposing the nuclear localization signals on the NF-kappa B heterodimer. Cyclooxygenase-2 (COX-2), which mediates prostaglandin synthesis during inflammation, is induced by oxidative stress mediated by NF-kappa B. We investigated whether the NF-kappa B signaling pathway affected cell death and COX-2 expression after hypoxia-induced oxidative stress in wild-type (WT) and copper/zinc-superoxide dismutase transgenic (SOD1 Tg) astrocytes. In WT astrocytes, phospho-I kappa B alpha was highly expressed after oxygen-glucose deprivation (OGD) and 2 h of reperfusion, concomitant with the decrease in I kappa B alpha. The NF-kappa B p50 level increased similarly in WT and SOD1 Tg astrocytes (1.2-/1.4-fold) after OGD. Electrophoretic mobility shift assay showed higher DNA-binding activity of NF-kappa B p50 in WT than in SOD1 Tg astrocytes 6 h after 4 h of OGD. The COX-2 level was induced by 2.7- and 1.3-fold after OGD in WT and SOD1 Tg astrocytes, and an antioxidant protected both groups against OGD injury. Superoxide dismutase transgenic cells were 23% more protective against OGD injury than WTs when assessed by lactate dehydrogenase release. Ho
- Files in This Item
-
Go to Link
- Appears in
Collections - 약학대학 > 약학부 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.