Gene expression profiling in PKD2 overexpressed and PKD2/c-myc co-overexpressed HEK 293 cells

Abstract

Polycystic kidney diseases are characterized by the progressive expansion of multiple cystic lesions, which compromise the function of normal parenchyma. There have been major advances in the study of the pathophysiology of cyst formation and the progression of renal failure in ADPKD. Tubular epithelial hyperplasia, tubular fluid secretion, and alterations in extracellular matrix were characterized in ADPKD. Overexpression pattern of c-myc in association with increased proliferation and apoptosis of tubular epithelial cells might be similar to a molecular feature of early and late stages of human ADPKD. Based on the evidence that c-myc is a major mediator of cystogenesis, we had established pkd2, and c-myc and pkd2 overexpressed HEK293 cell line. Gene expression profiles were compared with plasmid overexpressed HEK 293 cells line components, pkd2 overexpressed HEK 293 cell line components, and c-myc and pkd2 overexpressed HEK 293 cell line components by cDNA microarray technology. Among the approximately 7,700 genes that were analyzed, we identified novel genes that might be related to cystogenesis from gene expression profiles.