IL-18 enhances the migration ability of murine melanoma cells through the generation of ROI and the MAPK pathway
- Authors
- Jung, Min Kyung; Song, Hyun Keun; Kim, Kyung-Eun; Hur, Dae Young; Kim, Taesung; Bang, Saic; Park, Hyunjeong; Cho, Dae Ho
- Issue Date
- Nov-2006
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- IL-18; melanoma; migration; ROI; MAPK
- Citation
- IMMUNOLOGY LETTERS, v.107, no.2, pp 125 - 130
- Pages
- 6
- Journal Title
- IMMUNOLOGY LETTERS
- Volume
- 107
- Number
- 2
- Start Page
- 125
- End Page
- 130
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/15042
- DOI
- 10.1016/j.imlet.2006.08.004
- ISSN
- 0165-2478
1879-0542
- Abstract
- Interleukin-18 (IL-18) has multiple effects on various cells that are involved in immune escape of murine melanoma cells and in the inflammatory responses. This study investigated the effect of IL-18 on the ability of murine melanoma cells to migrate by using B16F10 cells and the IL-18 antisense transfectants of B16F10 cells (the B16F10/IL-18 antisense transfectant). The B16F10 cells were more able to migrate than were the B16F10/IL-18 antisense transfectants. An exogenous IL-18 treatment improved the ability of the B16F10/IL-18 antisense transfectant cells to migrate, indicating that IL-18 enhanced the migration ability of melanoma cells. To determine the signaling mechanisms involved in IL-18enhanced migration, we measured the ROI levels. It was found that the ROI levels were increased by IL-18, and an antioxidant, N-acetyl-L-cystein (NAC) blocked the effect of IL-18 on migration, suggesting the involvement of ROI in the signal transduction of IL-18-enhanced cell migration. IL-18-enhanced cell migration was also reduced by PD98059. In addition, the level of ERK1/2 phosphorylation was markedly increased by treating with exogenous IL-18 at 20min. These results suggest that IL-18 enhances the ability of melanoma cells to migrate via the generation of ROI and the MAPK pathway. (c) 2006 Elsevier B.V. All rights reserved.
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Collections - 대학 > 기초교양대학 > 기초교양학부 > 1. Journal Articles
- 원격대학원 > 향장미용학과 > 1. Journal Articles
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