O2 variant chip to simulate site-specific skeletogenesis from hypoxic bone marrowopen access
- Authors
- Kim, Hye-Seon; Ha, Hyun-Su; Kim, Dae-Hyun; Son, Deok Hyeon; Baek, Sewoom; Park, Jeongeun; Lee, Chan Hee; Park, Suji; Yoon, Hyo-Jin; Yu, Seung Eun; Kang, Jeon Il; Park, Kyung Min; Shin, Young Min; Lee, Jung Bok; Sung, Hak-Joon
- Issue Date
- Mar-2023
- Publisher
- American Association for the Advancement of Science
- Citation
- Science advances, v.9, no.12
- Journal Title
- Science advances
- Volume
- 9
- Number
- 12
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/151947
- DOI
- 10.1126/sciadv.add4210
- ISSN
- 2375-2548
2375-2548
- Abstract
- The stemness of bone marrow mesenchymal stem cells (BMSCs) is maintained by hypoxia. The oxygen level increases from vessel-free cartilage to hypoxic bone marrow and, furthermore, to vascularized bone, which might direct the chondrogenesis to osteogenesis and regenerate the skeletal system. Hence, oxygen was diffused from relatively low to high levels throughout a three-dimensional chip. When we cultured BMSCs in the chip and implanted them into the rabbit defect models of low-oxygen cartilage and high-oxygen calvaria bone, (i) the low oxygen level (base) promoted stemness and chondrogenesis of BMSCs with robust antioxidative potential; (ii) the middle level (two times ≥ low) pushed BMSCs to quiescence; and (iii) the high level (four times ≥ low) promoted osteogenesis by disturbing the redox balance and stemness. Last, endochondral or intramembranous osteogenesis upon transition from low to high oxygen in vivo suggests a developmental mechanism-driven solution to promote chondrogenesis to osteogenesis in the skeletal system by regulating the oxygen environment.
- Files in This Item
-
Go to Link
- Appears in
Collections - 이과대학 > 생명시스템학부 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.