동충하초(Cordyceps militaris) 균사체 액체발효 포스트바이오틱스로부터 항염증 활성다당 분리
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김연숙 | - |
dc.contributor.author | 신현영 | - |
dc.contributor.author | 김훈 | - |
dc.contributor.author | 정은진 | - |
dc.contributor.author | 김현경 | - |
dc.contributor.author | 서민근 | - |
dc.contributor.author | 서형주 | - |
dc.contributor.author | 유광원 | - |
dc.date.accessioned | 2023-11-08T06:52:09Z | - |
dc.date.available | 2023-11-08T06:52:09Z | - |
dc.date.issued | 2023-02 | - |
dc.identifier.issn | 1225-4339 | - |
dc.identifier.issn | 2287-4992 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/152084 | - |
dc.description.abstract | To investigate the anti-inflammatory activity of submerged culture using Cordyceps militaris mycelium, culture-including mycelia was extracted and lyophilized into postbiotics (hot-water extract; CM-HW). HW was fractionated into crude polysaccharide (CM-CP) by ethanol precipitation, and CM-CP was further dialyzed into CM-DCP by dialysis with running water using 12~14 kDa dialysis tube. When the cytotoxicity of subfractions against cells was assessed, no subfraction had a cytotoxic impact that was substantially different from the control groups. In an inflammatory model using LPS-stimulated RAW 264.7 cells, CM-DCP significantly decreased IL-6 and MCP-1 production levels compared to the LPS-control group. CM-DCP also inhibited IL-6 and IL-8 secretion in HaCaT keratinocytes stimulated with TNF-α and IFN-γ. In the meanwhile, the neutral sugar content and mannose ratio of anti-inflammatory CM-DCP were higher than the other fractions, and CM-DCP contained β-1,3/1,6-glucan of 216.1 mg/g. High pressure size exclusion chromatography revealed that CM-DCP contained molecules with a molecular weight range of 5.6 to 144.0 kDa. In conclusion, postbiotics of C. militaris mycelium significantly promoted anti-inflammatory activity, suggesting that neutral polysaccharides including Glc and Man contribute to the anti-inflammation in RAW 264.7 or HaCaT cells. | - |
dc.format.extent | 11 | - |
dc.language | 한국어 | - |
dc.language.iso | KOR | - |
dc.publisher | 한국식품영양학회 | - |
dc.title | 동충하초(Cordyceps militaris) 균사체 액체발효 포스트바이오틱스로부터 항염증 활성다당 분리 | - |
dc.title.alternative | Anti-Inflammatory Active Polysaccharide from Postbiotics of Cordyceps militaris Mycelium-Liquid Culture | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.9799/ksfan.2023.36.1.006 | - |
dc.identifier.bibliographicCitation | 한국식품영양학회지, v.36, no.1, pp 6 - 16 | - |
dc.citation.title | 한국식품영양학회지 | - |
dc.citation.volume | 36 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 6 | - |
dc.citation.endPage | 16 | - |
dc.identifier.kciid | ART002934000 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordAuthor | Cordyceps militaris | - |
dc.subject.keywordAuthor | liquid culture | - |
dc.subject.keywordAuthor | postbiotics | - |
dc.subject.keywordAuthor | anti-inflammatory activity | - |
dc.subject.keywordAuthor | polysaccharide | - |
dc.identifier.url | https://ksfn.kr/html/sub4_04.html | - |
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