동충하초(Cordyceps militaris) 균사체 액체발효 포스트바이오틱스로부터 항염증 활성다당 분리Anti-Inflammatory Active Polysaccharide from Postbiotics of Cordyceps militaris Mycelium-Liquid Culture
- Other Titles
- Anti-Inflammatory Active Polysaccharide from Postbiotics of Cordyceps militaris Mycelium-Liquid Culture
- Authors
- 김연숙; 신현영; 김훈; 정은진; 김현경; 서민근; 서형주; 유광원
- Issue Date
- Feb-2023
- Publisher
- 한국식품영양학회
- Keywords
- Cordyceps militaris; liquid culture; postbiotics; anti-inflammatory activity; polysaccharide
- Citation
- 한국식품영양학회지, v.36, no.1, pp 6 - 16
- Pages
- 11
- Journal Title
- 한국식품영양학회지
- Volume
- 36
- Number
- 1
- Start Page
- 6
- End Page
- 16
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/152084
- DOI
- 10.9799/ksfan.2023.36.1.006
- ISSN
- 1225-4339
2287-4992
- Abstract
- To investigate the anti-inflammatory activity of submerged culture using Cordyceps militaris mycelium, culture-including mycelia was extracted and lyophilized into postbiotics (hot-water extract; CM-HW). HW was fractionated into crude polysaccharide (CM-CP) by ethanol precipitation, and CM-CP was further dialyzed into CM-DCP by dialysis with running water using 12~14 kDa dialysis tube. When the cytotoxicity of subfractions against cells was assessed, no subfraction had a cytotoxic impact that was substantially different from the control groups. In an inflammatory model using LPS-stimulated RAW 264.7 cells, CM-DCP significantly decreased IL-6 and MCP-1 production levels compared to the LPS-control group. CM-DCP also inhibited IL-6 and IL-8 secretion in HaCaT keratinocytes stimulated with TNF-α and IFN-γ. In the meanwhile, the neutral sugar content and mannose ratio of anti-inflammatory CM-DCP were higher than the other fractions, and CM-DCP contained β-1,3/1,6-glucan of 216.1 mg/g. High pressure size exclusion chromatography revealed that CM-DCP contained molecules with a molecular weight range of 5.6 to 144.0 kDa. In conclusion, postbiotics of C. militaris mycelium significantly promoted anti-inflammatory activity, suggesting that neutral polysaccharides including Glc and Man contribute to the anti-inflammation in RAW 264.7 or HaCaT cells.
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