Single metal-organic framework-embedded nanopit arrays: A new way to control neural stem cell differentiationopen access
- Authors
- Cho, Yeon-Woo; Jee, Seohyeon; Suhito, Intan Rosalina; Lee, Jeong-Hyeon; Park, Chun Gwon; Choi, Kyung Min; Kim, Tae-Hyung
- Issue Date
- Apr-2022
- Publisher
- AMER ASSOC ADVANCEMENT SCIENCE
- Citation
- SCIENCE ADVANCES, v.8, no.16
- Journal Title
- SCIENCE ADVANCES
- Volume
- 8
- Number
- 16
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/152841
- DOI
- 10.1126/sciadv.abj7736
- ISSN
- 2375-2548
2375-2548
- Abstract
- Stable and continuous supply of essential biomolecules is critical to mimic in vivo microenvironments wherein spontaneous generation of various cell types occurs. Here, we report a new platform that enables highly efficient neuronal cell generation of neural stem cells using single metal-organic framework (MOF) nanoparticle-embedded nanopit arrays (SMENA). By optimizing the physical parameters of homogeneous periodic nanopatterns, each nanopit can confine single nMOFs (UiO-67) that are specifically designed for long-term storage and release of retinoic acid (RA). The SMENA platform successfully inhibited physical interaction with cells, which contributed to remarkable stability of the nMOF (RA subset of UiO-67) structure without inducing nanoparticle-mediated toxicity issues. Owing to the continuous and long-term supply of RA, the neural stem cells showed enhanced mRNA expressions of various neurogenesis-related activities. The developed SMENA platform can be applied to other stem cell sources and differentiation lineages and is therefore useful for various stem cell-based regenerative therapies.
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