Analysis of human plasma proteome by 2DE-and 2D nanoLC-based mass spectrometry
- Authors
- Choi, KS; Song, LG; Park, YM; Marshall, J; Lund, AL; Shion, H; Park, EM; Chae, HZ; Park, JH
- Issue Date
- Feb-2006
- Publisher
- TAYLOR & FRANCIS INC
- Keywords
- plasma proteome; immunoaffinity depletion; 2DE; 2DLC; mass spectrometry
- Citation
- PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, v.36, no.1, pp 3 - 17
- Pages
- 15
- Journal Title
- PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
- Volume
- 36
- Number
- 1
- Start Page
- 3
- End Page
- 17
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/15432
- DOI
- 10.1080/10826060500388447
- ISSN
- 1082-6068
1532-2297
- Abstract
- We compared the 2DE coupled to MALDI-TOF-MS and ESI-MS/MS analysis (2DE-MS) and the on-line 2D nanoLC, followed by nanoESI-MS/MS analysis (2DLC-MS), for the separation and identification of proteins in high abundance protein-depleted human plasma. Identification of proteins in the plasma by the two methods demonstrated that the majority of the identified protein set was unique to each method. Therefore, if a comprehensive coverage of the proteome identification is desired, it is ideal to apply both methods. The 2DE-MS method is amenable to protein spot-based quantitation, whereas the 2DLC-MS method may provide an advantage of the high throughput application.
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