Mancozeb이 마우스 비장세포의 IFN-g 생성능에 미치는 영향

Abstract

Mancozeb(MCZ), a polymeric complex of zinc and manganese salts of ethylene bisthiocarbamate, is widelyused in agriculture as fungicides, insecticides, and herbicides. MCZ can be occupationally and environmentallytoxicity of MCZ on immune responses has not been systematically investigated. We investigated the effects ofMCZ exposure on the activities of murine splenocytes through evaluation of splenocytes cellularity and INF-γsynthesis. Splenocytes were examned ex vivofrom mice orally treated with various doses of MCZ for 1 day(acute exposure, 2,500, 5,000, 10,000 mg/kg) or for 5 consecutive days per week for 4 weeks (subacuteIL-2. Splenocytes from naive mice were cultured with various concentration of MCZ (50, 500, 1,000 ng/mL)in the presence of Con A or PHA plus IL-2 for 2 days in vitro. IFN-γproduction was decreased with the invitro exposure to all concentration of MCZ. The spleen cellularity and IFN-γproduction by splenocytes fromMCZ-acutely and -subacutely exposured mice were decreased in comparision with that of control group.