In Vitro 에서 Bisphenol A 노출에 의한 세포성 면역반응의 변화

Abstract

In order to investigate the effects of bisphenol A (BPA) on cell mediated immune reaction in vitro we examined the allogenic mixed lymphocyte reaction (MLR), splenocytes proliferation (SP) to T cell mitogens and IFN-g production. Splenocytes of Balb/c mice (1.5?05 cells/well) were co-cultured with different numbers of mitomycin C-treated mature dentritic cells (DCs) in presence of BPA (25, 50, 100 mM) and [3H]thymidine incorporation (cpm) was measured by scintilation counting. Splenocytes (2?06 cells/well) were cultured with mitogens, Con A (2 mg/ml), PHA (5 mg/ml) and IL-2 (0.1 mg/ml), or PMA (5 ng/ml) and INO (1 mg/ml) in presence of BPA (1, 10, 25, 50, 100 mM) and SP was assessed by MTT assay. IFN-g levels in culture supernant were determined by ELISA. At low concentration, BPA slightly increased MLR, SP and IFN-g levels, but at higher concentration it showed significant inhibitory effects on these immunological parameters. These results indicate that BPA is able to alternate cell-mediated immune reaction.