Conditionally immortalized syncytiotrophoblast cell lines as new tools for study of the blood-placenta barrier
- Authors
- Kitano, T; Iizasa, H; Hwang, IW; Hirose, Y; Morita, T; Maeda, T; Nakashima, E
- Issue Date
- Jun-2004
- Publisher
- PHARMACEUTICAL SOC JAPAN
- Keywords
- TR-TBTs; blood-placenta barrier; transporter
- Citation
- BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.27, no.6, pp 753 - 759
- Pages
- 7
- Journal Title
- BIOLOGICAL & PHARMACEUTICAL BULLETIN
- Volume
- 27
- Number
- 6
- Start Page
- 753
- End Page
- 759
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/15875
- DOI
- 10.1248/bpb.27.753
- ISSN
- 0918-6158
1347-5215
- Abstract
- Syncytiotrophoblasts play an essential role in restriction of drug delivery through the blood-placenta barrier (BPB). Conditionally immortalized syncytiotrophoblast cell lines, TR-TBTs, were established at gestational day 18 from pregnant transgenic rats (Tg-rats) harboring the temperature-sensitive SV 40 (ts SV40) large T-antigen. TR-TBTs exhibit temperature-sensitive cell growth due to the expression of SV 40 large T-antigen, and thus the cell growth can be regulated by changing the culture temperature. TR-TBTs exhibit typical properties of syncytiotrophoblast cells, such as syncytium-like morphology, the expression of cytokeratins and hormones, and polarized expression of glucose transporter 1 (GLUT1) and GLUT3. TR-TBTs express in vivo influx and efflux transporters, such as taurine transporter (TauT), betaine/GABA transporter (BGT-1), amino acid transporter 2 (ATA2), organic anion transporting polypeptide 2 (oatp2), organic cation/carnitine transporter (OCTN2), P-glycoprotein (P-gp), and breast cancer resistance protein (BCRP/ABCG2). Moreover, TR-TBTs exhibit taurine, GABA, and DHEA-S uptake activity via TauT, BGT-1, and oatp2, respectively. Therefore, TR-TBTs can be used for the analysis of these functions and would be a good in vitro models for investigating carrier-mediated transport functions at the BPB.
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