Dual-Emissive Detection of ATP and Hypochlorite Ions for Monitoring Inflammation-Driven Liver Injury In Vitro and In Vivo
DC Field | Value | Language |
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dc.contributor.author | Fortibui, Maxine Mambo | - |
dc.contributor.author | Park, Chaewon | - |
dc.contributor.author | Kim, Na Yoon | - |
dc.contributor.author | Kim, Tae Hyun | - |
dc.contributor.author | Lee, Min Hee | - |
dc.date.accessioned | 2024-06-27T04:30:20Z | - |
dc.date.available | 2024-06-27T04:30:20Z | - |
dc.date.issued | 2024-05 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.issn | 1520-6882 | - |
dc.identifier.uri | https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/160230 | - |
dc.description.abstract | Reactive oxygen species play a pivotal role in liver disease, contributing to severe liver damage and chronic inflammation. In liver injury driven by inflammation, adenosine-5′-triphosphate (ATP) and hypochlorite ion (ClO-) emerge as novel biomarkers, reflecting mitochondrial dysfunction and amplified oxidative stress, respectively. However, the dynamic fluctuations of ATP and ClO- in hepatocytes and mouse livers remain unclear, and multidetection techniques for these biomarkers are yet to be developed. This study presents RATP-NClO, a dual-channel fluorescent bioprobe capable of synchronously detecting ATP and ClO- ions. RATP-NClO exhibits excellent selectivity and sensitivity for ATP and ClO- ions, demonstrating a dual-channel fluorescence response in a murine hepatocyte cell line. Upon intravenous administration, RATP-NClO reveals synchronized ATP depletion and ClO- amplification in the livers of mice with experimental metabolic dysfunction-associated steatohepatitis (MASH). Through a comprehensive analysis of the principal mechanism of the developed bioprobe and the verification of its reliable detection ability in both in vitro and in vivo settings, we propose it as a unique tool for monitoring changes in intracellular ATP and ClO- level. These findings underscore its potential for practical image-based monitoring and functional phenotyping of MASH pathogenesis. © 2024 American Chemical Society. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | American Chemical Society | - |
dc.title | Dual-Emissive Detection of ATP and Hypochlorite Ions for Monitoring Inflammation-Driven Liver Injury In Vitro and In Vivo | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1021/acs.analchem.4c00270 | - |
dc.identifier.scopusid | 2-s2.0-85194357676 | - |
dc.identifier.wosid | 001234439500001 | - |
dc.identifier.bibliographicCitation | Analytical Chemistry, v.96, no.23, pp 9408 - 9415 | - |
dc.citation.title | Analytical Chemistry | - |
dc.citation.volume | 96 | - |
dc.citation.number | 23 | - |
dc.citation.startPage | 9408 | - |
dc.citation.endPage | 9415 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | REACTIVE OXYGEN | - |
dc.subject.keywordPlus | HEPATIC STEATOSIS | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | TOXICITY | - |
dc.subject.keywordPlus | ISCHEMIA | - |
dc.subject.keywordPlus | CELLS | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/acs.analchem.4c00270 | - |
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