Dual-Emissive Detection of ATP and Hypochlorite Ions for Monitoring Inflammation-Driven Liver Injury In Vitro and In Vivo
- Authors
- Fortibui, Maxine Mambo; Park, Chaewon; Kim, Na Yoon; Kim, Tae Hyun; Lee, Min Hee
- Issue Date
- May-2024
- Publisher
- American Chemical Society
- Citation
- Analytical Chemistry, v.96, no.23, pp 9408 - 9415
- Pages
- 8
- Journal Title
- Analytical Chemistry
- Volume
- 96
- Number
- 23
- Start Page
- 9408
- End Page
- 9415
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/160230
- DOI
- 10.1021/acs.analchem.4c00270
- ISSN
- 0003-2700
1520-6882
- Abstract
- Reactive oxygen species play a pivotal role in liver disease, contributing to severe liver damage and chronic inflammation. In liver injury driven by inflammation, adenosine-5′-triphosphate (ATP) and hypochlorite ion (ClO-) emerge as novel biomarkers, reflecting mitochondrial dysfunction and amplified oxidative stress, respectively. However, the dynamic fluctuations of ATP and ClO- in hepatocytes and mouse livers remain unclear, and multidetection techniques for these biomarkers are yet to be developed. This study presents RATP-NClO, a dual-channel fluorescent bioprobe capable of synchronously detecting ATP and ClO- ions. RATP-NClO exhibits excellent selectivity and sensitivity for ATP and ClO- ions, demonstrating a dual-channel fluorescence response in a murine hepatocyte cell line. Upon intravenous administration, RATP-NClO reveals synchronized ATP depletion and ClO- amplification in the livers of mice with experimental metabolic dysfunction-associated steatohepatitis (MASH). Through a comprehensive analysis of the principal mechanism of the developed bioprobe and the verification of its reliable detection ability in both in vitro and in vivo settings, we propose it as a unique tool for monitoring changes in intracellular ATP and ClO- level. These findings underscore its potential for practical image-based monitoring and functional phenotyping of MASH pathogenesis. © 2024 American Chemical Society.
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