Vitamin C downregulates interleukin-18 production by increasing reactive oxygen intermediate and mitogen-activated protein kinase signalling in B16F10 murine melanoma cells
- Authors
- Cho, D; Hahm, E; Kang, JS; Kim, YI; Yang, Y; Park, JH; Kim, D; Kim, SH; Kim, YS; Hur, DY; Park, H; Pang, S; Hwang, YI; Lee, WJ
- Issue Date
- Dec-2003
- Publisher
- LIPPINCOTT WILLIAMS & WILKINS
- Keywords
- interleukin-18; vitamin C; melanoma; reactive oxygen intermediates; p38; mitogen-activated protein kinase
- Citation
- MELANOMA RESEARCH, v.13, no.6, pp 549 - 554
- Pages
- 6
- Journal Title
- MELANOMA RESEARCH
- Volume
- 13
- Number
- 6
- Start Page
- 549
- End Page
- 554
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16145
- DOI
- 10.1097/00008390-200312000-00002
- ISSN
- 0960-8931
1473-5636
- Abstract
- We recently reported that interleukin-18 (IL-18) is highly expressed in malignant skin tumours such as melanomas, and may play a key role in the malignancy of such tumours. This study was designed to investigate the mechanisms of IL-18 regulation by vitamin C in B16F10 murine melanoma cells. Cells were treated with vitamin C, and the expression of IL-18 was measured by reverse transcription-polymerase chain reaction and intracellular flow cytometry analysis. Decreased IL-18 production and a significant reduction in IL-18 mRNA transcript were detected in cells treated with vitamin C. The effect of vitamin C treatment was blocked by the antioxidant N-acetyl-L-cysteine, suggesting that vitamin C affects IL-18 expression by up-regulating intracellular reactive oxygen intermediate (ROI) levels. To investigate whether the mitogen-activated protein kinase (MAPK) signalling pathway is involved in the downregulation of IL-18 production, cells were pretreated with SB203580, an inhibitor of p38 MAPK, prior to the addition of vitamin C. This pretreatment blocked the decrease in IL-18 production. However, vitamin C treatment enhanced the expression of phosphorylated p38 MAPK. Taken together, we conclude that vitamin C increases intracellular ROI levels, and regulates IL-18 production through the MAPK signalling pathway. (C) 2003 Lippincott Williams Wilkins.
- Files in This Item
-
Go to Link
- Appears in
Collections - 이과대학 > 생명시스템학부 > 1. Journal Articles
- 대학 > 기초교양대학 > 기초교양학부 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16145)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.