Detailed Information

Cited 0 time in webofscience Cited 11 time in scopus
Metadata Downloads

Cloning and expression of alkaline phosphatase gene from Schizosaccharomyces pombe

Authors
Kang, SWCho, YWPark, EHAhn, KSLim, CJ
Issue Date
May-2001
Publisher
SPRINGER-VERLAG SINGAPORE PTE LTD
Keywords
alkaline phosphatase; cDNA; fission yeast; genomic DNA; Schizosaccharomyces pombe
Citation
JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.34, no.3, pp 262 - 267
Pages
6
Journal Title
JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY
Volume
34
Number
3
Start Page
262
End Page
267
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16657
ISSN
1225-8687
Abstract
A cDNA coding alkaline phosphatase (AP) homologue mas isolated from a cDNA library of Schizosaccharomyces pombe by colony hybridization. The nucleotide sequence of the cloned cDNA appeared to lack the N-terminal coding region, The genomic DNA encoding alkaline phosphatase homologue was isolated from S. pombe chromosomal DNA using PCR, The amplified DNA fragment was ligated into plasmid pRS315 to generate the recombinant plasmid pSW20. The DNA insert was subcloned as two smaller fragments for nucleotide sequencing. The sequence contains 2,789 bp and encodes a protein of 532 amino acids with a molecular mass of 58,666 daltons. The S, pombe cells containing plasmid pSW20 showed much higher AP activity compared with the yeast cells with vector only. This indicates that the cloned AP gene apparently encodes AP. The predicted amino acid sequence of the S, pombe AP shares homology with those of other known APs.
Files in This Item
Go to Link
Appears in
Collections
약학대학 > 약학부 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE