Novel Associations between Related Proteins and Cellular Effects of High-Density Lipoproteinopen access
- Authors
- Choi, Seungbum; Park, Yae Eun; Cheon, Eun Jeong; Kim, Kyeong Yeon; Kim, Miso; Ann, Soo-jin; Noh, Hye-Min; Lee, Jaeho; Lee, Chan Joo; Lee, Seung-Taek; Lee, Cheolju; Lee, Ji Eun; Lee, Sang-Hak
- Issue Date
- Mar-2020
- Publisher
- KOREAN SOC CARDIOLOGY
- Keywords
- Atherosclerosis; Cardiovascular diseases; Proteomics; Lipoproteins; Immunity
- Citation
- KOREAN CIRCULATION JOURNAL, v.50, no.3, pp 236 - 247
- Pages
- 12
- Journal Title
- KOREAN CIRCULATION JOURNAL
- Volume
- 50
- Number
- 3
- Start Page
- 236
- End Page
- 247
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/2495
- DOI
- 10.4070/kcj.2019.0195
- ISSN
- 1738-5520
1738-5555
- Abstract
- Background and Objectives: Recent studies have examined the structure-function relationship of high-density lipoprotein (HDL). This study aimed to identify and rank HDL-associated proteins involved in several biological function of HDL. Methods: HDLs isolated from 48 participants were analyzed. Cholesterol efflux capacity, effect of HDL on nitric oxide production, and vascular cell adhesion molecule-1 expression were assessed. The relative abundance of identified proteins in the highest vs. lowest quartile was expressed using the normalized spectral abundance factor ratio. Results: After adjustment by multiple testing, six proteins, thyroxine-binding globulin, alpha-1B-glycoprotein, plasma serine protease inhibitor, vitronectin, angiotensinogen, and serum amyloid A-4, were more abundant (relative abundance ratio >= 2) in HDLs with the highest cholesterol efflux capacity. In contrast, three proteins, complement C4-A, alpha-2-macroglobulin, and immunoglobulin mu chain C region, were less abundant (relative abundance ratio <0.5). In terms of nitric oxide production and vascular cell adhesion molecule-1 expression, no proteins showed abundance ratios >= 2 or <0.5 after adjustment. Proteins correlated with the functional parameters of HDL belonged to diverse biological categories. Conclusions: In summary, this study ranked proteins showing higher or lower abundance in HDLs with high functional capacities and newly identified multiple proteins linked to cholesterol efflux capacity.
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