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High-depth fluorescence imaging using a two-photon FRET system for mitochondrial pH in live cells and tissues
- Authors
- Chang, Min Jung; Kim, Kyutae; Park, Kyun Seob; Kang, Ji Su; Lim, Chang Su; Kim, Hwan Myung; Kang, Chulhun; Lee, Min Hee
- Issue Date
- Dec-2018
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- CHEMICAL COMMUNICATIONS, v.54, no.96, pp 13531 - 13534
- Pages
- 4
- Journal Title
- CHEMICAL COMMUNICATIONS
- Volume
- 54
- Number
- 96
- Start Page
- 13531
- End Page
- 13534
- ISSN
- 1359-7345
1364-548X
- Abstract
- We developed a fluorescent pH probe (1) capable of two-photon excitation and far-visible-emission based on FRET, composed of naphthalimide-piperazine-rhodamine. It exhibited a pH-dependent reversible and fast ratiometric fluorescence change in the rhodamine emission. Probe 1 was applied to image the pH perturbations of mitochondria in living cells and tissues.
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