Development of suspension cell culture model to mimic circulating tumor cellsopen access
- Authors
- Park, Ji Young; Jeong, Ae Lee; Joo, Hyun Jeong; Han, Sora; Kim, So-Hyun; Kim, Hye-Youn; Lim, Jong-Seok; Lee, Myeong-Sok; Choi, Hyung-Kyoon; Yang, Young
- Issue Date
- Jan-2018
- Publisher
- IMPACT JOURNALS LLC
- Keywords
- suspension cells; metastasis; lipidomic profile; metabolic profile
- Citation
- ONCOTARGET, v.9, no.1, pp 622 - 640
- Pages
- 19
- Journal Title
- ONCOTARGET
- Volume
- 9
- Number
- 1
- Start Page
- 622
- End Page
- 640
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/4767
- DOI
- 10.18632/oncotarget.23079
- ISSN
- 1949-2553
1949-2553
- Abstract
- Circulating tumor cells (CTCs) are essential for the establishment of distant metastasis. Numerous studies have characterized CTCs as metastatic precursors; however, the molecular nature of CTCs has not been completely revealed yet due to the low number of CTCs in the blood stream. As an alternative approach, we developed a long-term suspension cell culture model using human breast cancer cell lines to mimic CTCs. We found that more than 40 passaged suspension cells acquired the ability to enhance metastasis like cancer stem cells. To identify molecular changes acquired during the suspension cell culture, we analyzed metabolic and lipidomic profiles as well as transcriptome in MDA-MB-468 suspension cells. Glutamate and leucine levels increased in suspension cells, and cholesterol synthesis pathway was altered. The inhibition of glutamate metabolic pathway decreased the proliferation of suspension cells compared to that of adherent cells. In the lipidomic profile, PC species containing long chain and polyunsaturated fatty acids increased in suspension cells and these species could be authentic and specific biomarkers for highly metastatic cancers. As this CTC-mimicking suspension cell culture model may easily apply to various types of cancer, we suggest this model as a great tool to develop therapeutic targets and drugs to eradicate metastatic cancer cells.
- Files in This Item
-
Go to Link
- Appears in
Collections - 이과대학 > 생명시스템학부 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.