In Vivo Expression of EphrinA5-Fc in Mice Results in Cephalic Neural Crest Agenesis and Craniofacial Abnormalities
- Authors
- Noh, Hyuna; Park, Eunjeong; Park, Soochul
- Issue Date
- Jan-2014
- Publisher
- KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
- Keywords
- cephalic neural crest; early brain development; EphA; ephrin-A
- Citation
- MOLECULES AND CELLS, v.37, no.1, pp 59 - 65
- Pages
- 7
- Journal Title
- MOLECULES AND CELLS
- Volume
- 37
- Number
- 1
- Start Page
- 59
- End Page
- 65
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/11000
- DOI
- 10.14348/molcells.2014.2279
- ISSN
- 1016-8478
0219-1032
- Abstract
- Eph receptors and their ligands ephrins have been implicated in guiding the directed migration of neural crest cells (NCCs). In this study, we found that Wnt1-Cre-mediated expression of ephrinA5-Fc along the dorsal midline of the dien-and mesencephalon resulted in severe craniofacial malformation of mouse embryo. Interestingly, expression of cephalic NCC markers decreased significantly in the frontonasal process and branchial arches 1 and 2, which are target areas for the migratory cephalic NCCs originating in the dien-and mesencephalon. In addition, these craniofacial tissues were much smaller in mutant embryos expressing ephrinA5-Fc. Importantly, EphA7-positive cephalic NCCs were absent along the dorsal dien-and mesencephalon of mutant embryos expressing ephrinA5-Fc, suggesting that the generation of cephalic NCCs is disrupted due to ephrinA5-Fc expression. NCC explant experiments suggested that ephrinA5-Fc perturbed survival of cephalic NCC precursors in the dorsal midline tissue rather than affecting their migratory capacity, which was consistent with our previous report that expression of ephrinA5-Fc in the dorsal midline is responsible for severe neuroepithelial cell apoptotic death. Taken together, our findings strongly suggest that expression of ephrinA5-Fc decreases a population of cephalic NCC precursors in the dorsal midline of the dien-and mesencephalon, thereby disrupting craniofacial development in the mouse embryos.
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