Activation-Tagged Suppressors of a Weak Brassinosteroid Receptor Mutant
- Authors
- Kang, Bin; Wang, Hao; Nam, Kyoung Hee; Li, Jiayang; Li, Jianming
- Issue Date
- Jan-2010
- Publisher
- CELL PRESS
- Keywords
- Brassinosteroid; BRI1; bri1-301; auxin; YUCCA
- Citation
- MOLECULAR PLANT, v.3, no.1, pp 260 - 268
- Pages
- 9
- Journal Title
- MOLECULAR PLANT
- Volume
- 3
- Number
- 1
- Start Page
- 260
- End Page
- 268
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/13296
- DOI
- 10.1093/mp/ssp099
- ISSN
- 1674-2052
1752-9867
- Abstract
- Brassinosteroids (BRs) are important plant hormones that act synergistically with auxin to regulate a variety of plant developmental and physiological processes. In the past decade, genetic and biochemical studies have revealed a linear signaling pathway that relies on protein phosphorylation to transmit the BR signal into the nucleus, altering expression of hundreds of genes to promote plant growth. We conducted an activation-tagging based suppressor screen to look for Arabidopsis genes that, when overexpressed by inserted 35S enhancer elements, could suppress the dwarf phenotype of a weak BR receptor mutant bri1-301. This screen identified a total of six dominant activation-tagged bri1 suppressors (atbs-Ds). Using a plasmid rescue approach, we discovered that the bri1-301 suppression effect in four atbs-D mutants (atbs3-D to atbs6-D) was caused by overexpression of a YUCCA gene thought to be involved in tryptophan-dependent auxin biosynthesis. Interestingly, the three activation-tagged YUCCA genes belong to the YUCCA IIA subfamily that includes two other members out of 11 known Arabidopsis YUCCA genes. In addition, our molecular studies revealed a T-DNA insertion near a basic helix-loop-helix gene in atbs1-D and a T-DNA insertion in a region carrying a BR biosynthetic gene in atbs2-D. Further studies of these atbs-D mutants could lead to better understanding of the BR signaling process and the BR-auxin interaction.
- Files in This Item
-
Go to Link
- Appears in
Collections - 이과대학 > 생명시스템학부 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.