Simultaneous Determination and Stability Test of Two Phthalic Anhydride Derivatives, Senkyunolide A and Z-Ligustilide, in the Water Extract of Cnidium Rhizome from Different Geographical Regions and Species Using HPLC-UVD Analysis
- Authors
- Jang, Hyeon Seok; Kang, Kyo Bin; Yoo, Guijae; Kwon, Euna; Yun, Jun-Won; Kang, Byeong-Cheol; Hong, In Kee; Kim, Seung Hyun; Sung, Sang Hyun; Cho, Hyun-Jong; Jang, Young Pyo; Yang, Heejung
- Issue Date
- Jun-2018
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- Cnidium officinale; High-performance liquid chromatography with an ultraviolet detector; Ligusticum chuanxiong; Simultaneous determination; Senkyunolide AZ-ligustilide
- Citation
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.39, no.6, pp 784 - 788
- Pages
- 5
- Journal Title
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY
- Volume
- 39
- Number
- 6
- Start Page
- 784
- End Page
- 788
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/146924
- DOI
- 10.1002/bkcs.11471
- ISSN
- 0253-2964
1229-5949
- Abstract
- Cnidium rhizome from Cnidium officinale Makino and Ligusticum chuanxiong Hort (Umbelliferae) is traditionally used to treat pain in East Asian countries. Senkyunolide A and Z-ligustilide are phthalic anhydride derivatives that are the bioactive components of cnidium rhizome and chemical markers of the two species; they are also susceptible to external stimuli. In this study, we developed a method to identify two chemical markers using high-performance liquid chromatography (HPLC) with an ultraviolet detector (UVD); the contents of senkyunolide A and Z-ligustilide in cnidium rhizome from different regions were quantified. The stabilities of the compounds were tested at two different temperatures over 12 months. An HPLC-UVD system was used to identify and quantify senkyunolide A and Z-ligustilide in 10 C. officinale samples and 6 L. chuanxiong samples from China and Korea. The long-term stability of the C. officinale samples in dark conditions at 4 and 25 degrees C was also tested. The calibration curves for the two chemical markers showed high linearity (R2 = 1.0000), the precision was high (relative standard deviation (RSD) <4.17%), and the recoveries were within the range of 95-105%. The contents were mostly independent of the geographical origin and species, and >10% of the compounds degraded after storage at
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