Chunghyul-dan acts as an anti-inflammatory agent in endothelial cells by regulating gene expression
- Authors
- 정우상; 조진구; 인경민; 김종민; 조기호; 박정미; 문상관; 김경욱; 박성욱; 피재호; 박상규; 정윤화; 박헌용; 고창남
- Issue Date
- Dec-2010
- Publisher
- 한국통합생물학회
- Citation
- Animal Cells and Systems, v.14, no.4, pp 275 - 282
- Pages
- 8
- Journal Title
- Animal Cells and Systems
- Volume
- 14
- Number
- 4
- Start Page
- 275
- End Page
- 282
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/147847
- DOI
- 10.1080/19768354.2010.525824
- ISSN
- 1976-8354
2151-2485
- Abstract
- Chunghyul-dan (CHD) is a combinatorial drug known to exert anti-inflammatory effects in endothelial cells. In this study, we employed global transcriptional profiling using cDNA microarrays to identify molecular mechanisms responsible for the anti-inflammatory activity of CHD in endothelial cells. An analysis of the microarray data revealed that transcript levels of monocyte chemotactic protein-1 (MCP-1), vascular cell-adhesion molecule-1 (VCAM-1) and activated leukocyte cell-adhesion molecule were dramatically altered in CHD-treated endothelial cells. These changes in gene expression were confirmed by RT-PCR, Western blotting and ELISA. Chronic CHD treatment also appeared to decrease MCP-1 secretion, probably as a result of decreased MCP-1 expression. In addition, we determined that chronic CHD treatment inhibited lipopolysaccharide-stimulated adhesion of THP-1 leukocytes to endothelial cells. The inhibitory effect of CHD on LPS-stimulated adhesion resulted from down-regulation of VCAM-1 expression. Transmigration of THP-1 leukocytes through endothelial cells was also inhibited by chronic CHD treatment. In conclusion, CHD controls a variety of inflammatory activities by regulating MCP-1 and VCAM-1 gene expression.
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