Enhancement of ectopic bone formation by bone morphogenetic protein-2 delivery using heparin-conjugated PLGA nanoparticles with transplantation of bone marrow-derived mesenchymal stem cells
- Authors
- Kim S.E.; Jeon O.; Lee J.B.; Bae M.S.; Chun H.-J.; Moon S.-H.; Kwon I.K.
- Issue Date
- Nov-2008
- Publisher
- S. Karger AG
- Keywords
- Bone marrow-derived mesenchymal stem cell; Bone morphogenetic protein-2; Bone regeneration; Heparin-conjugated PLGA nanoparticles
- Citation
- Journal of Biomedical Science, v.15, no.6, pp 771 - 777
- Pages
- 7
- Journal Title
- Journal of Biomedical Science
- Volume
- 15
- Number
- 6
- Start Page
- 771
- End Page
- 777
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/148156
- DOI
- 10.1007/s11373-008-9277-4
- ISSN
- 1021-7770
1423-0127
- Abstract
- This study was performed to determine if a combination of previously undifferentiated bone marrow-derived mesenchymal stem cells (BMMSCs) and exogenous bone morphogenetic protein-2 (BMP-2) delivered via heparin-conjugated PLGA nanoparticles (HCPNs) would extensively regenerate bone in vivo. In vitro testing found that the HCPNs were able to release BMP-2 over a 2-week period. Human BMMSCs cultured in medium containing BMP-2-loaded HCPNs for 2 weeks differentiated toward osteogenic cells expressing alkaline phosphatase (ALP), osteopontin (OPN) and osteocalcin (OCN) mRNA, while cells without BMP-2 expressed only ALP. In vivo testing found that undifferentiated BMMSCs with BMP-2-loaded HCPNs induce far more extensive bone formation than either implantation of BMP-2-loaded HCPNs or osteogenically differentiated BMMSCs. This study demonstrates the feasibility of extensive in vivo bone regeneration by transplantation of undifferentiated BMMSCs and BMP-2 delivery via HCPNs. ?? 2008 National Science Council Taipei.
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