Assay of 2-naphthol in human urine by high-performance liquid chromatography
- Authors
- Kim, Heon; Kim, Yong-Dae; Lee, Holik; Kawamoto, Toshihiro; Yang, Mihi; Katoh, Takahiko
- Issue Date
- Nov-1999
- Publisher
- Elsevier BV
- Citation
- Journal of Chromatography B: Biomedical Sciences and Applications, v.734, no.2, pp 211 - 217
- Pages
- 7
- Journal Title
- Journal of Chromatography B: Biomedical Sciences and Applications
- Volume
- 734
- Number
- 2
- Start Page
- 211
- End Page
- 217
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/150173
- DOI
- 10.1016/S0378-4347(99)00350-3
- ISSN
- 1387-2273
- Abstract
- This paper describes a novel liquid chromatographic method for the quantitation of 2-naphthol in human urine. Urine samples were extracted after enzymatic hydrolysis of glucuronides and sulfates; 2-naphthol was then separated using reversed-phase high-performance liquid chromatography. The corresponding detection limits were 0.04 ng/ml for the standard sample in acetonitrile and 0.13 ng/ml for urine samples. The level of urinary 2-naphthol in 100 Korean shipyard workers was analyzed using this new method. The level ranged from 0.21 ng/ml (0.26 mu mol/mol creatinine) to 34.19 ng/ml (59.11 mu mol/mol creatinine), and the mean+/-standard deviation was 5.08 ng/ml (6.60 mu mol/mol creatinine)+/-5.75 ng/ml (9.22 mu mol/mol creatinine). The mean+/-standard deviation of urinary 2-naphthol level of smokers, 7.03 ng/ml (8.49 mu mol/mol creatinine)+/-6.16 ng/ml (10.23 mu mol/mol creatinine), was significantly higher than that of non-smokers, 2.49 ng/ml (4.10 mu mol/mol creatinine)+/-3.92 ng/ml (7.03 mu mol/mol creatinine). (C) 1999 Published by Elsevier Science BN. All rights reserved.
- Files in This Item
-
Go to Link
- Appears in
Collections - 약학대학 > 약학부 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.