Hydrogen peroxide activates p70(S6k) signaling pathway
- Authors
- Bae, GU; Seo, DW; Kwon, HK; Lee, HY; Hong, S; Lee, ZW; Ha, KS; Lee, HW; Han, JW
- Issue Date
- Nov-1999
- Publisher
- ELSEVIER
- Citation
- JOURNAL OF BIOLOGICAL CHEMISTRY, v.274, no.46, pp 32596 - 32602
- Pages
- 7
- Journal Title
- JOURNAL OF BIOLOGICAL CHEMISTRY
- Volume
- 274
- Number
- 46
- Start Page
- 32596
- End Page
- 32602
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/150177
- DOI
- 10.1074/jbc.274.46.32596
- ISSN
- 0021-9258
1083-351X
- Abstract
- We investigated a possible role of reactive oxygen species (ROS) in p70(S6k) activation, which plays an important role in the progression of cells from G(0)/G(1) to S phase of the cell cycle by translational up-regulation of a family of mRNA transcripts that encode for components of the protein synthetic machinery. Treatment of mouse epidermal cell JB6 with H2O2 generated extracellularly by glucose/glucose oxidase led to the activation of p70(S6k) and p90(Rsk) and to phosphorylation of p42(MAPK)/p44(MAPK) The activation of p70(S6k) and p90(Rsk) was dose-dependent and transient, maximal activities being in extracts treated for 15 and 30 min, respectively. Further characterization of ROS-induced activation of p70S6k using specific inhibitors for p70S6k Signaling pathway, rapamycin, and wortmannin revealed that ROS acted upstream of the rapamycin-sensitive component FRAP/RAFT and wortmannin-sensitive component phosphatidylinositol 3-kinase, because both inhibitors caused the inhibition of ROS-induced p70(S6k) activity. In addition, Ca2+ chelation also inhibited ROS-induced activation of p70S6k, indicating that Ca2+ is a mediator of p70(S6k) activation by ROS. However, down-regulation of 12-O-tetradecanoylphorbol-13-acetate (TPA)-responsive protein kinase C (PKC) by chronic pretreatment with TPA or a specific PHC in
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