Preparation of gamma-linolenic acid rich triacylglycerol from borage oil via a two-step lipase-catalyzed reaction
- Authors
- Yoon, Hui Su; Lee, Yu Jin; Kim, Byung Hee; Kim, Yangha; Kim, In-Hwan
- Issue Date
- 1-Mar-2023
- Publisher
- WILEY
- Keywords
- borage oil; Candida rugosa lipase; enrichment; glyceride; triglyceride (TAG); gamma-linolenic acid (GLA)
- Citation
- JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, v.100, no.3, pp 215 - 224
- Pages
- 10
- Journal Title
- JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
- Volume
- 100
- Number
- 3
- Start Page
- 215
- End Page
- 224
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/151955
- DOI
- 10.1002/aocs.12678
- ISSN
- 0003-021X
1558-9331
- Abstract
- gamma-Linolenic acid (GLA) rich triacylglycerol (TAG) was successfully synthesized from glyceride, instead of glycerol, and fatty acid (FA) via Lipozyme TL IM-catalyzed esterification as a novel strategy. In the first step, GLA was enriched into glyceride fraction from borage oil by Candida rugosa lipase-catalyzed hydrolysis. The glyceride was separated from the reaction mixture by molecular distillation. GLA was enriched from 20.64% in borage oil to 45.94% in the glyceride fraction under optimum conditions. In the second step, the Lipozyme TL IM-catalyzed synthesis of TAG was carried out with the glyceride, and the FA obtained by saponification of a portion of the glyceride. The optimum conditions were the temperature of 50?, the enzyme loading of 10%, and the vacuum level of 20 mmHg, respectively. The maximum TAG content of approximately 92% was achieved after 12 h under the optimum conditions.
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