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조골세포에 있어서 형질전환 효율의 최적화Optimization of Transfection Efficiency in Osteoblastic Cells
- Other Titles
- Optimization of Transfection Efficiency in Osteoblastic Cells
- Issue Date
- Feb-2006
- Publisher
- 숙명여자대학교 약학연구소
- Citation
- 약학논문집-숙명여자대학교, v.23, pp 55 - 58
- Journal Title
- 약학논문집-숙명여자대학교
- Volume
- 23
- Start Page
- 55
- End Page
- 58
- ISSN
- 1225-3723
- Abstract
- Achievement of optimal transfection efficiency is invaluable for mechanistic studies on osteoblastic gene regulation. In the present study, we have shown that the efficiency of biochemical transfection reagents varies significantly in UAMS-32 osteoblastic cells. The highest transfection efficiency was obtained when 4 mg DNA and Welfect-plus ™ was used in UAMS-32 osteoblastic cells. We also showed the evidence of the applicability of this method for the efficient transfeption of osteoblastic cells with reporter constructs for luciferase reporter assay.
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