Electrochemical Impedance-Based Biosensors for the Label-Free Detection of the Nucleocapsid Protein from SARS-CoV-2open access
- Authors
- Cho, Hana; Shim, Suhyun; Cho, Won Woo; Cho, Sungbo; Baek, Hanseung; Lee, Sang-Myung; Shin, Dong-Sik
- Issue Date
- Jun-2022
- Publisher
- AMER CHEMICAL SOC
- Keywords
- electrochemical sensor; nucleocapsid protein; SARS-CoV-2; impedance; label-free detection
- Citation
- ACS SENSORS, v.7, no.6, pp 1676 - 1684
- Pages
- 9
- Journal Title
- ACS SENSORS
- Volume
- 7
- Number
- 6
- Start Page
- 1676
- End Page
- 1684
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/152728
- DOI
- 10.1021/acssensors.2c00317
- ISSN
- 2379-3694
- Abstract
- Diagnosis of coronavirus disease (COVID-19) is important because of the emergence and global spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Real-time polymerase chain reaction (PCR) is widely used to diagnose COVID-19, but it is time-consuming and requires sending samples to test centers. Thus, the need to detect antigens for rapid on-site diagnosis rather than PCR is increasing. We quantified the nucleocapsid (N) protein in SARS-CoV-2 using an electro-immunosorbent assay (El-ISA) and a multichannel impedance analyzer with a 96-interdigitated microelectrode sensor (ToAD). The El-ISA measures impedance signals from residual detection antibodies after sandwich assays and thus offers highly specific, label-free detection of the N protein with low cross-reactivity. The ToAD sensor enables the real-time electrochemical detection of multiple samples in conventional 96-well plates. The limit of detection for the N protein was 0.1 ng/mL with a detection range up to 10 ng/mL. This system did not detect signals for the S protein. While this study focused on detecting the N protein in SARS-CoV-2, our system can also be widely applicable to detecting various biomolecules involved in antigen-antibody interactions.
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