Efficient induction of T helper type 1-mediated immune responses in antigen-primed mice by anti-CD3 single-chain Fv/interleukin-18 fusion DNA
- Authors
- Kim, EJ; Cho, D; Kim, TS
- Issue Date
- Jan-2004
- Publisher
- WILEY
- Citation
- IMMUNOLOGY, v.111, no.1, pp 27 - 34
- Pages
- 8
- Journal Title
- IMMUNOLOGY
- Volume
- 111
- Number
- 1
- Start Page
- 27
- End Page
- 34
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/15909
- DOI
- 10.1111/j.1365-2567.2004.01784.x
- ISSN
- 0019-2805
1365-2567
- Abstract
- Two types of T helper (Th) cells - Th1 and Th2 - play different roles in protection and immunopathology. The Th1 cell-mediated immune response plays an important role in inducing the host defence against intracellular bacteria and also in cancer immunotherapy. To effectively induce Th1 immune responses, we constructed a mammalian expression plasmid (pAnti-CD3sFv/IL-18) carrying a fusion gene in which anti-CD3 single-chain Fv (sFv) cDNA, the smallest unit of antibody recognizing the CD3 epsilon moiety of the T-cell receptor, was covalently linked to mature interleukin (IL)-18 cDNA. Intramuscular injection of ovalbumin (OVA)-sensitized BALB/c mice with pAnti-CD3sFv/IL-18 DNA efficiently increased the production of both OVA-specific interferon-gamma and anti-OVA immunoglobulin G2a, compared to injection with pAnti-CD3sFv DNA. In addition, pAnti-CD3sFv/IL-18 was more efficient than a mixture of pAnti-CD3sFv + pIL-18 in inducing OVA-specific, Th1 immune responses and also in inhibiting OVA-specific, IL-4 production. These studies indicate that vaccination with pAnti-CD3sFv/IL-18 fusion DNA efficiently induces the Th1 immune response in antigen-sensitized mice.
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