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Identification and characterization of a novel (S)-ketoprofen-specific esterase
- Issue Date
- Jun-2007
- Publisher
- Elsevier BV
- Citation
- International Journal of Biological Macromolecules, v.41, no.1, pp 1 - 7
- Pages
- 7
- Journal Title
- International Journal of Biological Macromolecules
- Volume
- 41
- Number
- 1
- Start Page
- 1
- End Page
- 7
- ISSN
- 0141-8130
1879-0003
- Abstract
- A new (S)-ketoprofen specific esterase (EST-Y29) was identified from a metagenome library from environmental samples, which showed homologies with class C-beta lactamase, penicillin binding protein, and other lipases/esterases. In order to investigate the biochemical and biophysical properties, the recombinant protein was overexpressed, purified to homogeneity, and characterized. This EST-Y29 has high catalytic activity against p-nitrophenyl esters of short fatty acids (C-2 and C-4) and alpha-naphthyl acetate with activation energy of 30.40/mol. We have further characterized EST-Y29 using high performance liquid chromatography (HPLC), circular dichroism (CD), dynamic light scattering (DLS) and size exclusion chromatography (SEC). (c) 2006 Published by Elsevier B.V.
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