Concentration of docosahexsaenoic acid from tuna oil by a combination of solvent crystallization and lipase-catalyzed ethanolysis
- Authors
- Park, Mi Soon; Wee, Chido; Lee, Junsoo; Kim, Byung Hee; Kim, Hak-Ryul; Kim, In-Hwan
- Issue Date
- Jul-2024
- Publisher
- WILEY
- Keywords
- docosahexaenoic acid; enrichment; ethanolysis; lipase; Lipozyme RM IM; solvent crystallization
- Citation
- JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, v.101, no.7, pp 637 - 645
- Pages
- 9
- Journal Title
- JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
- Volume
- 101
- Number
- 7
- Start Page
- 637
- End Page
- 645
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/160318
- DOI
- 10.1002/aocs.12817
- ISSN
- 0003-021X
1558-9331
- Abstract
- The docosahexaenoic acid (DHA) was concentrated from tuna oil fatty acid using solvent crystallization combined with lipase-catalyzed ethanolysis. In the first step, solvent crystallization was carried out to concentrate DHA from tuna oil fatty acid using acetonitrile as a solvent. The optimal conditions of solvent crystallization were the crystallization temperature of -40 degrees C and the fatty acid to solvent ratio of 1:8 (w/v). This step increased the DHA content in the original tuna oil fatty acid from 22% up to 61%. In the second step, lipase-catalyzed ethanolysis was conducted with DHA-enriched fatty acid from the first step using Lipozyme RM IM (from Rhizomucor miehei) as a biocatalyst. The optimum conditions of this second step were the reaction temperature of 20 degrees C and the molar ratio of 1:1 (fatty acid to ethanol). Overall, DHA enrichment with purity of 85% was obtained by the two step processes.
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