Pap1-dependent regulation of the GSTII gene from the fission yeast
- Authors
- Lim, CJ; Cho, YW; Sa, JH; Lim, HW; Kim, HG; Kim, SJ; Park, EH
- Issue Date
- Dec-2002
- Publisher
- SPRINGER-VERLAG SINGAPORE PTE LTD
- Keywords
- fission yeast; glutathione S-transferase; oxidative stress; Pap1; regulation; Schizosaccharomyces pombe
- Citation
- MOLECULES AND CELLS, v.14, no.3, pp 431 - 436
- Pages
- 6
- Journal Title
- MOLECULES AND CELLS
- Volume
- 14
- Number
- 3
- Start Page
- 431
- End Page
- 436
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/16369
- ISSN
- 1016-8478
0219-1032
- Abstract
- The genomic DNA encoding a second glutathione S-transferase (GSTII) was previously isolated from the fission yeast Schizosaccharomyces pombe. Its expression was shown to be induced by menadione, mercuric chloride, o-dinitrobenzene, and NO-generating S-nitroso-N-acetylpenicillamine using the GSTII-lacZ fusion harboring the 910 bp upstream region from the translational initiation point. In this study, the additional fusion plasmids pGST50-590 and pGST50-6R590 were constructed to carry the 590 bp upstream region in the vectors YEp357 and YEp367R, respectively. The synthesis of P-galactosidase from the fusion plasmid pGST50-590 was about 3-fold higher than that from the fusion plasmid pGST50-F, indicating the presence of negatively activating sequence in the -910 similar to -590 region. It was also enhanced by the same agents, which induced the synthesis of beta-galactosidase from the fusion plasmid pGST50-F. The synthesis of beta-galactosidase from both fusion plasmids pGST50-F and pGST50-590 was enhanced by the overexpressed Pap1 protein. The synthesis of P-galactosidase from the two YEp367R derivatives pGST50-6R-F and pGST50-6R-590 was greatly decreased in the Pap1-negative strain TP108-3C. These results propose the Pap1-dependent regulation of the GSTII gene from the fission yeast.
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