Substrate selectivity of Novozym 435 in the esterification of glycerol with an equimolar mixture of linoleic, conjugated linoleic, and pinolenic acids
- Authors
- 김병희; 우현준; 김지원; 김인환; 최희돈; 최인욱
- Issue Date
- Jun-2016
- Publisher
- Wiley - V C H Verlag GmbbH & Co.
- Citation
- European Journal of Lipid Science and Technology, v.118, no.6, pp 928 - 937
- Pages
- 10
- Journal Title
- European Journal of Lipid Science and Technology
- Volume
- 118
- Number
- 6
- Start Page
- 928
- End Page
- 937
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/3356
- DOI
- 10.1002/ejlt.201500363
- ISSN
- 1438-7697
1438-9312
- Abstract
- The selectivity of Novozym 435, an immobilized Candida antarctica lipase B toward linoleic (LA), conjugated linoleic (CLA), and pinolenic acids (PLA) was investigated in the esterification of glycerol with an equimolar (27.3 mol% each) mixture of the fatty acids (FAs) in a solvent‐free system to prepare triacylglycerols (TAGs) with anti‐obesity effects. The maximal TAG content of 98.9 wt% was achieved under the following conditions: temperature, 70°C; reaction time, 24 h; glycerol‐to‐FA mixture molar ratio, 1:3; enzyme loading, 10 wt% of total substrates; vacuum level, 0.4 kPa. During the initial phase of the reaction, Novozym 435 showed pronounced selectivity in the order of PLA > CLA > LA; however, no significant difference in the selectivity of Novozym 435 toward the three FAs was observed after the reaction plateaued. Novozym 435 showed the significant selectivity toward PLA over CLA and LA at the sn‐2 position of TAGs throughout the reaction. The resulting TAGs had the same total content (∼27 mol% each) of the three FAs. However, the LA, CLA, and PLA contents at the sn‐2 position were 27.7, 24.7, and 30.3 mol%, respectively, indicating that unlike LA, CLA, and PLA were unevenly distributed on the glycerol backbone of the TAGs.
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