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Substrate selectivity of Novozym 435 in the esterification of glycerol with an equimolar mixture of linoleic, conjugated linoleic, and pinolenic acids

Authors
김병희우현준김지원김인환최희돈최인욱
Issue Date
Jun-2016
Publisher
Wiley - V C H Verlag GmbbH & Co.
Citation
European Journal of Lipid Science and Technology, v.118, no.6, pp 928 - 937
Pages
10
Journal Title
European Journal of Lipid Science and Technology
Volume
118
Number
6
Start Page
928
End Page
937
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/3356
DOI
10.1002/ejlt.201500363
ISSN
1438-7697
1438-9312
Abstract
The selectivity of Novozym 435, an immobilized Candida antarctica lipase B toward linoleic (LA), conjugated linoleic (CLA), and pinolenic acids (PLA) was investigated in the esterification of glycerol with an equimolar (27.3 mol% each) mixture of the fatty acids (FAs) in a solvent‐free system to prepare triacylglycerols (TAGs) with anti‐obesity effects. The maximal TAG content of 98.9 wt% was achieved under the following conditions: temperature, 70°C; reaction time, 24 h; glycerol‐to‐FA mixture molar ratio, 1:3; enzyme loading, 10 wt% of total substrates; vacuum level, 0.4 kPa. During the initial phase of the reaction, Novozym 435 showed pronounced selectivity in the order of PLA > CLA > LA; however, no significant difference in the selectivity of Novozym 435 toward the three FAs was observed after the reaction plateaued. Novozym 435 showed the significant selectivity toward PLA over CLA and LA at the sn‐2 position of TAGs throughout the reaction. The resulting TAGs had the same total content (∼27 mol% each) of the three FAs. However, the LA, CLA, and PLA contents at the sn‐2 position were 27.7, 24.7, and 30.3 mol%, respectively, indicating that unlike LA, CLA, and PLA were unevenly distributed on the glycerol backbone of the TAGs.
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