icaA Gene of Staphylococcus aureus Responds to NaCl, Leading to Increased Biofilm Formation
- Authors
- Lee, Soomin; Kim, Sejeong; Lee, Heeyoung; Ha, Jimyeong; Lee, Jeeyeon; Choi, Yukyung; Oh, Hyemin; Yoon, Yohan; Choi, Kyoung-Hee
- Issue Date
- Mar-2018
- Publisher
- INT ASSOC FOOD PROTECTION
- Keywords
- Biofilm; icaA; NaCl; Staphylococcus aureus
- Citation
- JOURNAL OF FOOD PROTECTION, v.81, no.3, pp 412 - 416
- Pages
- 5
- Journal Title
- JOURNAL OF FOOD PROTECTION
- Volume
- 81
- Number
- 3
- Start Page
- 412
- End Page
- 416
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/4623
- DOI
- 10.4315/0362-028X.JFP-17-238
- ISSN
- 0362-028X
1944-9097
- Abstract
- The objective of this study was to elucidate the role of the icaA gene in biofilm formation of Staphylococcus aureus exposed to NaCl. The icaA-deletion mutant of S. aureus ATCC 13565 was constructed with the temperature-sensitive plasmid pIMAY. Microtiter plate assays were performed to confirm biofilm formation for both the wild type and the mutant at 0% (control), 2, 4, and 6% NaCl. The microtiter plate assay revealed that biofilm formation by the wild type increased (P < 0.05) as NaCl concentration increased, but biofilm formation of the mutant was not affected by NaCl concentration. Biofilm formation by the mutant was lower (P < 0.05) than that by the wild type. These results indicate that icaA plays an important role in biofilm formation by S. aureus when the pathogen is exposed to NaCl.
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