Nanowire Aptasensors for Electrochemical Detection of Cell-Secreted Cytokines
- Authors
- Liu, Ying; Rahimian, Ali; Krylyuk, Sergiy; Vu, Tam; Crulhas, Bruno; Stybayeva, Gulnaz; Imanbekova, Meruyert; Shin, Dong-Sik; Davydov, Albert; Revzin, Alexander
- Issue Date
- Nov-2017
- Publisher
- AMER CHEMICAL SOC
- Keywords
- electrochemical biosensor; IFN-gamma aptamer; nanowire electrode; aptasensor; tuberculosis detection
- Citation
- ACS SENSORS, v.2, no.11, pp 1644 - 1652
- Pages
- 9
- Journal Title
- ACS SENSORS
- Volume
- 2
- Number
- 11
- Start Page
- 1644
- End Page
- 1652
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/5052
- DOI
- 10.1021/acssensors.7b00486
- ISSN
- 2379-3694
- Abstract
- Cytokines are small proteins secreted by immune cells in response to pathogens/infections; therefore, these proteins can be used in diagnosing infectious diseases. For example, release of a cytokine interferon (IFN)-gamma from T-cells is used for blood-based diagnosis of tuberculosis (TB). Our lab has previously developed an atpamer-based electrochemical biosensor for rapid and sensitive detection of IFN-gamma. In this study, we explored the use of silicon nanowires (NWs) as a way to create nanostructured electrodes with enhanced sensitivity for IFN-gamma. Si NWs were covered with gold and were further functionalized with thiolated aptamers specific for IFN-gamma. Aptamer molecules were designed to form a hairpin and in addition to terminal thiol groups contained redox reporter molecules methylene blue. Binding of analyte to aptamer-modified NWs (termed here nanowire aptasensors) inhibited electron transfer from redox reporters to the electrode and caused electrochemical redox signal to decrease. In a series of experiments we demonstrate that NW aptasensors responded 3x faster and were 2x more sensitive to IFN-gamma compared to standard flat electrodes. Most significantly, NW aptasensors allowed detection of IFN-gamma from as few as 150 T-cells/mL while ELISA did not pick up signal from the same number of cells. One of the challenges faced by ELISA-based TB diagnostics is poor performance in patients whose T-cell numbers are low, typically HIV patients. Therefore, NW aptasensors developed here may be used in the future for more sensitive monitoring of IFN-gamma responses in patients coinfected with HIV/TB.
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