An electrochemical aptasensor for detection of bovine interferon gamma
- Authors
- Crulhas, Bruno P.; Hadley, Dustin; Liu, Ying; Shin, Dong-Sik; Stybayeva, Gulnaz; Imanbekova, Meruyert; Hill, Ashley E.; Pedrosa, Valber; Revzin, Alexander
- Issue Date
- Aug-2017
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- ANALYTICAL METHODS, v.9, no.31, pp 4527 - 4532
- Pages
- 6
- Journal Title
- ANALYTICAL METHODS
- Volume
- 9
- Number
- 31
- Start Page
- 4527
- End Page
- 4532
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/8228
- DOI
- 10.1039/c7ay01313b
- ISSN
- 1759-9660
1759-9679
- Abstract
- Bovine tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) in which it is hard to identify the pathological symptoms. Release of bovine interferon gamma (BoIFN-gamma) by T-cells provides an important diagnostic marker of MTB infections. In this work, we developed for the first time an electrochemical aptasensor for sensitive and specific determination of BoIFN-gamma. A thiolated IFN-gbinding aptamer was conjugated with methylene blue (MB) and immobilized on a gold electrode by selfassembly. Binding of IFN-g to the electrode surface caused a conformational change in the aptamer, decreasing electron transfer efficiency. The redox current was quantified using square wave voltammetry (SWV) and was found to be specific for bovine IFN-g with detection limit of 0.1 nM in pristine buffer and 0.9 nM in blood. The biosensor described here may, in the future, be used for on-site testing of bovine blood to help better identify and contain outbreaks of bovine TB.
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