Soybean saponin inhibits tumor cell metastasis by modulating expressions of MMP-2, MMP-9 and TIMP-2
- Authors
- Kang, Ji-Hye; Han, In-Hee; Sung, Mi-Kyung; Yoo, Hoon; Kim, Young-Gyun; Kim, Jeong-Sang; Kawada, Teruo; Yu, Rina
- Issue Date
- 8-Mar-2008
- Publisher
- ELSEVIER IRELAND LTD
- Keywords
- soybean saponin; metastasis; MMP-2; MMP-9; inflammation
- Citation
- CANCER LETTERS, v.261, no.1, pp 84 - 92
- Pages
- 9
- Journal Title
- CANCER LETTERS
- Volume
- 261
- Number
- 1
- Start Page
- 84
- End Page
- 92
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/14299
- DOI
- 10.1016/j.canlet.2007.11.006
- ISSN
- 0304-3835
1872-7980
- Abstract
- The antimetastatic properties of soybean saponin were investigated by evaluating matrix metalloproteinase (MMP-2 and MMP-9) production in HT-1080 cells. The mRNA expression levels of MMP-2 and MMP-9 were determined by RT-PCR analysis. The levels of secreted MMP-2, MMP-9 and tissue inhibitor of metalloproteinase-2 (TIMP-2) were determined by gelatin zymography and/or ELISA. The invasion of a Matrigel-coated membrane by human fibrosarcoma, HT-1080 and HT-29 colon cancer cells was quantitatively assessed by counting the migrated cells. The treatment of HT-1080 cells with soybean saponin inhibited the mRNA expression of and reduced the amounts of secreted MMP-2 and MMP-9, whereas it increased the amount of secreted TIMP-2 dose-dependently. Soybean saponin significantly inhibited the invasion of HT-1080 cells through a Matrigel-coated membrane. The antimetastatic properties by soybean saponin were further confirmed by in vivo mice experiment via the tail vein injection of CT-26 colon cancer cells after feeding the mice the dietary soybean saponin. The incidence of metastatic tumor colonization of lungs of mice moderately decreased 2 weeks after the tail vein injection of CT-26 cells. Our current data support the notion that soybean saponin inhibits tumor cell metastasis by suppressing MMP-2 and MMP-9 productions, and stimulating TIMP-2 secretion, thereby suggesting that soybean saponin has a chemopreventive property against cancer metastasis. (C) 2007 Elsevier Ireland Ltd. All rights reserved.
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