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Production of vanillin by metabolically engineered Escherichia coli

Authors
Yoon, SHLi, CKim, JELee, SHYoon, JYChoi, MSSeo, WTYang, JKKim, JYKim, SW
Issue Date
Nov-2005
Publisher
SPRINGER
Keywords
Ferulic acid; Metabolic engineering; Recombinant E. coli; Vanillin
Citation
BIOTECHNOLOGY LETTERS, v.27, no.22, pp 1829 - 1832
Pages
4
Journal Title
BIOTECHNOLOGY LETTERS
Volume
27
Number
22
Start Page
1829
End Page
1832
URI
https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/148829
DOI
10.1007/s10529-005-3561-4
ISSN
0141-5492
1573-6776
Abstract
E. coli was metabolically engineered to produce vanillin by expression of the fcs and ech genes from Amycolatopsis sp. encoding feruloyl-CoA synthetase and enoyl-CoA hydratase/aldolase, respectively. Vanillin production was optimized by leaky expression of the genes, under the IPTG-inducible trc promoter, in complex 2YT medium. Supplementation with glucose, fructose, galactose, arabinose or glycerol severely decreased vanillin production. The highest vanillin production of 1.1 g l(-1) was obtained with cultivation for 48 h in 2YT medium with 0.2% (w/v) ferulate, without IPTG and no supplementation of carbon sources.
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