Production of vanillin by metabolically engineered Escherichia coli
- Authors
- Yoon, SH; Li, C; Kim, JE; Lee, SH; Yoon, JY; Choi, MS; Seo, WT; Yang, JK; Kim, JY; Kim, SW
- Issue Date
- Nov-2005
- Publisher
- SPRINGER
- Keywords
- Ferulic acid; Metabolic engineering; Recombinant E. coli; Vanillin
- Citation
- BIOTECHNOLOGY LETTERS, v.27, no.22, pp 1829 - 1832
- Pages
- 4
- Journal Title
- BIOTECHNOLOGY LETTERS
- Volume
- 27
- Number
- 22
- Start Page
- 1829
- End Page
- 1832
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/148829
- DOI
- 10.1007/s10529-005-3561-4
- ISSN
- 0141-5492
1573-6776
- Abstract
- E. coli was metabolically engineered to produce vanillin by expression of the fcs and ech genes from Amycolatopsis sp. encoding feruloyl-CoA synthetase and enoyl-CoA hydratase/aldolase, respectively. Vanillin production was optimized by leaky expression of the genes, under the IPTG-inducible trc promoter, in complex 2YT medium. Supplementation with glucose, fructose, galactose, arabinose or glycerol severely decreased vanillin production. The highest vanillin production of 1.1 g l(-1) was obtained with cultivation for 48 h in 2YT medium with 0.2% (w/v) ferulate, without IPTG and no supplementation of carbon sources.
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Collections - 문과대학 > 문화관광외식학부 > 1. Journal Articles
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