Efficient Synthesis of Stearidonic Acid Enriched Triacylglycerol from Ahiflower Seed Oil via a Two-Step Enzyme Reactionopen access
- Authors
- Ju, Changhwan; Lee,Yu Jin; Yoon, Hui Su; Kim, Byung Hee; Kim, In-Hwan
- Issue Date
- Nov-2022
- Publisher
- Japan Oil Chemists Society
- Keywords
- ahiflower seed oil; Candida rugosa; Eversa® transform 2.0; stearidonic acid; triacylglycerol
- Citation
- Journal of Oleo Science, v.71, no.11, pp 1679 - 1688
- Pages
- 10
- Journal Title
- Journal of Oleo Science
- Volume
- 71
- Number
- 11
- Start Page
- 1679
- End Page
- 1688
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/152330
- DOI
- 10.5650/jos.ess22215
- ISSN
- 1345-8957
1347-3352
- Abstract
- Stearidonic acid (SDA) is a plant-based n-3 polyunsaturated fatty acid with multiple biological activities. The enrichment of SDA and synthesis of triacylglycerol (TAG) were carried out consecutively via two lipase-catalyzed reactions, hydrolysis, and esterification. First, SDA was enriched into a glyceride fraction from ahiflower seed oil by Candida rugosa lipase-catalyzed hydrolysis. Under the optimum conditions of 35℃, 0.1% lipase powder of Lipase OF, and 50% buffer solution (based on the weight of total substrate), SDA was enriched from 21.6 to 40.7 wt% in glyceride fraction. SDA-enriched TAG was then synthesized from the SDA-enriched glyceride and the SDA-enriched fatty acid via esterification using an in-house immobilized lipase as a biocatalyst. The SDA-enriched fatty acid was obtained from part of the SDA-enriched glyceride by saponification and the in-house immobilized lipase was prepared from Eversa® Transform 2.0 using Lewatit VP OC 1600 as a carrier. The optimum reaction conditions for the synthesis of TAG were a temperature of 50℃, an enzyme loading of 10%, and a vacuum of 10 mmHg. A maximum conversion to TAG of ca. 94% was achieved after 12 h under the optimum conditions. © 2022 by Japan Oil Chemists’ Society.
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