Immobilized Candida antarctica lipase B as an sn-1,3 regiospecific biocatalyst for the interesterification of triacylglycerols with fatty acid ethyl estersImmobilized Candida antarctica lipase B as an sn-1,3 regiospecific biocatalyst for the interesterification of triacylglycerols with fatty acid ethyl esters
- Other Titles
- Immobilized Candida antarctica lipase B as an sn-1,3 regiospecific biocatalyst for the interesterification of triacylglycerols with fatty acid ethyl esters
- Authors
- Hwang, Jihyun; Aum, Jiae; Lee, Seong Jae; Mun, Jeong Min; Kim, Seung Wook; Chung, Min‑Yu; Kim, In‑Hwan; Kim, Byung Hee
- Issue Date
- Jan-2024
- Publisher
- The Korean Society of Food Science and Technology
- Keywords
- Candida antarctica Lipase B; Immobilization; Interesterification; Lipozyme 435; Sn-1,3 specificity
- Citation
- Food Science and Biotechnology, v.33, no.1, pp 159 - 170
- Pages
- 12
- Journal Title
- Food Science and Biotechnology
- Volume
- 33
- Number
- 1
- Start Page
- 159
- End Page
- 170
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/159747
- DOI
- 10.1007/s10068-023-01337-7
- ISSN
- 1226-7708
2092-6456
- Abstract
- Candida antarctica lipase B (CALB) is regarded as non-regiospecific. This study aimed to investigate the regiospecificity of CALB in the solvent-free interesterification of high-oleic sunflower oil with stearic acid ethyl ester for 1,3-distearoyl-2-oleoylglycerol (SOS)-rich fat preparation using a packed bed reactor. The content ratio of 1,2-distearoyl-3-oleoylglycerol (SSO) to SOS (denoted by SSO/SOS content) obtained using Lipozyme 435 (a commercially immobilized CALB; 0–4.1%), at residence times (1–32 min) was similar to that obtained using Lipozyme RM IM (0–3.0%), but lower than that obtained using Lipozyme TL IM (6.0–39.4%). When immobilized on Lewatit VP OC 1600, Lipozyme CALB had an SSO/SOS content of 0–10.4%, which was greater than that of Palatase 20,000 L (0–1.1%) but was lower than that of Lipozyme TL 100 L (8.8–97.7%). Our findings suggest that immobilized CALB shows distinct sn-1,3 regiospecificity in the interesterification of triacylglycerol with fatty acid ethyl esters.
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