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백작약으로부터 식품부패미생물에 대한 항균성물질의 분리 및 동정Isolation and Identification of Antimicrobial Compound from Jakyak ( Paeonia japonica var. pilosa NAKAI )
- Other Titles
- Isolation and Identification of Antimicrobial Compound from Jakyak ( Paeonia japonica var. pilosa NAKAI )
- Issue Date
- Oct-2000
- Publisher
- 한국식품조리과학회
- Citation
- 한국식품조리과학회지, v.16, no.5, pp.67 - 74
- Journal Title
- 한국식품조리과학회지
- Volume
- 16
- Number
- 5
- Start Page
- 67
- End Page
- 74
- ISSN
- 2287-1780
- Abstract
- Antimicrobial activity of Jakyak(Paeonia japonica var. pilosa N_AKA1) was investigated. Methanol extract of dried Jakyak was fractionated to hexane, chloroform, ethylacetate, butanol and aqueous fraction. Ethylacetate fraction among these fractions showed the highest inhibitory effect on the microorganisms such as B. subilis, B. subtilis, L. monocytogenes, and V. parahaemolyticus at 500 ㎍/disc. Ethylacetate fraction was further fractionated into 11 fractions by silica gel column and thin layer chromatography(TLC). The results showed that ethylacetate fractions No. 3, 4 and 5 had the highest antimicrobial activity. They were mixed again, re-separated, and 5 fractions were obtained. Among them, the highest inhibitory effect was obtained in No. 3 fraction, which was identified as cetyl alcohol by HPLC and GC-MS.
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