Piperonal attenuates visceral adiposity in mice fed a high-fat diet: potential involvement of the adenylate cyclase-protein kinase A dependent pathway
- Authors
- Chu, Songyi; Narayan, Vikram P.; Sung, Mi-Kyung; Park, Taesun
- Issue Date
- Nov-2017
- Publisher
- WILEY
- Keywords
- Adenylate cyclase; Adipogenesis; Fatty acid oxidation; Piperonal; Protein kinase A
- Citation
- MOLECULAR NUTRITION & FOOD RESEARCH, v.61, no.11
- Journal Title
- MOLECULAR NUTRITION & FOOD RESEARCH
- Volume
- 61
- Number
- 11
- URI
- https://scholarworks.sookmyung.ac.kr/handle/2020.sw.sookmyung/5057
- DOI
- 10.1002/mnfr.201601124
- ISSN
- 1613-4125
1613-4133
- Abstract
- Scope: Piperonal is an aromatic compound found in vanilla and has a floral odor resembling vanillin. This study was aimed to test whether piperonal attenuates visceral adiposity induced by a high-fat diet (HFD) in mice and to explore the underlying molecular mechanisms. Methods and results: Male C57BL/6N mice were fed a normal diet, HFD, or 0.05% piperonal-supplemented HFD (PSD) for 10 weeks. PSD-fed mice showed attenuation of body weight gain, total visceral fat pad weights, and plasma lipid levels compared to HFD-fed mice. Piperonal supplementation of the HFD increased the mRNA expression of certain isotypes of adenylate cyclase (Adcy) and protein kinase A (PKA) in the white adipose tissue (WAT) of mice. The adipogenesis-related genes were downregulated, whereas fatty acid oxidation- and thermogenesis-related genes were upregulated in the WAT of PSD-fed mice compared to those in HFD-fed mice. Piperonal directly activated Adcy by decreasing the K-m for its substrate (ATP) in plasma membranes prepared from the WAT of mice. Furthermore, piperonal-induced inhibition of adipocyte differentiation and elevation of Adcy and PKA activities in 3T3-L1 cells were abrogated by an Adcy inhibitor. Conclusion: The anti-adipogenic effect of piperonal in mice fed the high-fat diet appears to be associated with increased Adcy-PKA signaling in WAT.
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