프로폴리스 섭취가 고지방 식이를 섭취한 흰쥐의 항산화능에 미치는 영향

Abstract

This study was performed to investigate the effect of propolis on phenotypic markers of antioxidative capacity in rats fed with high fat (HF) diet. Forty-five male Sprague-Dawley rats were divided into three groups and maintained for 8 weeks on experimental diets which were consisted of high-fat diet, high-fat diet supplemented with 1% (w/w) propolis, and high-fat diet supplemented with 5% (w/w) propolis. RBC and liver superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities were measured. Plasma concentrations of α-tocopherol and glutathione (GSH/GSSG) were determined and ferric reducing ability of plasma (FRAP) and randox-total antioxidant status (TAS) of plasma were evaluated. The concentration of plasma, liver and kidney malondialdehyde (MDA) were determined and lymphocyte DNA damage were also measured by comet assay. Study results indicated that RBC SOD activity and FRAP value were higher in HF＋1% propolis group compared to those of HF group, and liver MDA concentration and FRAP value of HF＋5% propolis were higher than those of animals fed with HF＋1% propolis and HF. Effect of propolis intake on GSH-Px, catalase, α-tocopherol, glutathione,and lymphocyte DNA damage were not significantly different among experimental groups. Liver weight was higher in HF＋5% propolis group compared to HF+1% propolis group or HF group, whereas kidney weight of HF＋5% propolis group was lowered than that of other two groups implying possible deleterious effects by high level supplementation. (Cancer Prev Res 15, 143-151, 2010)