남녀 노인층에서 Alzheimer 위험 예측 인자로써의Apo E4 Genotype에 관한 연구

Abstract

Recent studies described the E4 allele of apoE confers a two-to fourfold increased risk for late-onset Alzheimer's disease (LOAD), but LOAD pathology does not all fit neatly around apo E. Therefore, the goal of this study was to find the association between Alzheimer and apo E4 genotype in the 107 elderly between 50 to 64 years old who visited to FHWC of Sungshin Women's University. We conducted the questionnaire survey (general & 24 hr dietary recall), anthropometerics (BP, waist & BMI) and blood biochemistry (FBS & lipid profiles). LDL-c and HOMA-IR were calculated by Friedwald's and Matthew's formulas. The apo E genotyping was performed by PCR-RFLP method and subjects were divided into three allele groups (ε3; wild, ε2 & ε4; mutants). The apo E allele frequencies were 7.0% for the ε2, 83.6% for the ε3 and 9.3% for the ε4. In comparison with biochemistry characteristics by apo E genotype, FBS was significantly higher in E4 029.2 ± 6.8) than that in the others (ε2: 117 ± 7.4, ε3: 107.3 ± 2.2) (p ＜ 0.01). More than forty percents of E4 group shown the dyslipidemia [high TG (＞ 150 ㎎/㎗) & low HDL (＜ 40 ㎎/㎗: ♂ or ＜ 50 ㎎/㎗: ♀) ]. The cytokines levels such as IL-1 β, IL-6 and TNF - α were not different among three apoE alleles. After the adjusting sex, age & dietary fiber, LDL-c level was significantly higher in ε4 (108.3 ± 7.7) than that in ε2 (100.4 ± 8.4) (p ＜ 0.05). According to food intake and the recipe on the basis of 24 hr dietary recall, the elderly with ε4 allele took higher intake frequency of the light -colored vegetable (radish, onion & cabbage) and pan-fried foods (sauteed beef and vegetables, stir-fried vienna with vegetables) than the others. We knew that the elderly with ε 4 allele had been restricted the calories intakes with high dietary fiber (33.6+2.5 g/d) to maintain the normal level of FBS and LDL-c. On next study, the prevalence of Alzheimer's disease in this population who has E4 allele on the condition of calories restriction will be continually follow-up.